significant changes in the activity of gabatransaminase and succinate semialdehyde dehydrogenase of mouse hypothalamus following peripheral injection of cholecystokinin-8 and/or caerulein
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چکیده
the activities of 4-aminobutyric-2-oxoglutaric acid transaminase (gaba-t) and succinate semialdehyde dehydrogenase (ssadh) were determined in mouse hypothalamus after peripheral injections of cholecystokinin-8 (cck-x)and/or caerulein (cln). gaba transaminase activity was measured utilizing endogenous succinate semialdellyde dehydrogenase to convert the product of gab a-t, succinate semialdehyde, to succinate. the concurrently formed nadh was used as an estimate of gaba-t activity. similarly, the activity of ssadh was determined in terms of nadh. injection of cck-8 and/or cln inhibited the gaba-t and ssadh activities in dose-dependent responses. the activities of gab a-tand ssadh were diminished by 52 and 66 percent respectively, 30 minutes after injection of cck-8 (50 µg/kg body weight).similarly, peripheral injection of cln (50 µg/kg body weight) reduced the activity of gaba-t and ssadh by 56 and 65 percent respectively, in 30 minutes. using ill vitro models, the full activities of gaba-t and/ or ssadh, after incubation with cck-8 and/or cln, were restored following 30 hours of dialysis at 4°c. these results indicate direct and reversible interactions between the catabolizing enzymes of gaba (gaba-t and ssadh) and the peptides cck-8 and cln.
منابع مشابه
SIGNIFICANT CHANGES IN THE ACTIVITY OF GABATRANSAMINASE AND SUCCINATE SEMIALDEHYDE DEHYDROGENASE OF MOUSE HYPOTHALAMUS FOLLOWING PERIPHERAL INJECTION OF CHOLECYSTOKININ-8 AND/OR CAERULEIN
The activities of 4-aminobutyric-2-oxoglutaric acid transaminase (GABA-T) and succinate semialdehyde dehydrogenase (SSADH) were determined in mouse hypothalamus after peripheral injections of cholecystokinin-8 (CCK-X)and/or caerulein (CLN). GABA transaminase activity was measured utilizing endogenous succinate semialdellyde dehydrogenase to convert the product of GAB A-T, succinate semiald...
متن کاملSignificant Changes in the Activity of L-Glutamic Acid Decarboxylase of Mouse Hypothalamus After Peripheral Injection of Cholecystokinin-8 and Caerulein
The activity of one of the metabolizing enzymes of - aminobutyric acid, (GABA), was determined in mouse hypothalamus after peripheral injections of cholecystokinin-8 (CCK-8) and caerulein (CLN). The activity of this rate-limiting enzyme, L-glutamic acid decarboxylase, (GAD), did not change thirty minutes after peripheral injections of either CCK-8 or CLN in doses of 50g/kg body weight. Howeve...
متن کاملsignificant changes in the activity of l-glutamic acid decarboxylase of mouse hypothalamus after peripheral injection of cholecystokinin-8 and caerulein
the activity of one of the metabolizing enzymes of - aminobutyric acid, (gaba), was determined in mouse hypothalamus after peripheral injections of cholecystokinin-8 (cck-8) and caerulein (cln). the activity of this rate-limiting enzyme, l-glutamic acid decarboxylase, (gad), did not change thirty minutes after peripheral injections of either cck-8 or cln in doses of 50g/kg body weight. howeve...
متن کاملSignificant Changes in the Activity of L-glutamic Acid Decarboxylase of Mouse Hypothalamus after Peripheral Injection of Cholecystokinin-8 and Caerulein
The activity of one of the metabolizing enzymes of aminobutyric acid, (GABA), was determined in mouse hypothalamus after peripheral injections of cholecystokinin-8 (CCK-8) and caerulein (CLN). The activity of this rate-limiting enzyme, L-glutamic acid decarboxylase, (GAD), did not change thirty minutes after peripheral injections of either CCK-8 or CLN in doses of 50g/kg body weight. However,...
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عنوان ژورنال:
medical journal of islamic republic of iranجلد ۷، شماره ۴، صفحات ۲۶۳-۲۶۸
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